Sticky ends from different StyI sites may not be compatible.Ī C T G G G ( N ) 4 N T G A C C C ( N ) 4 SmaI can be used at 37☌ for brief incubations.Ĭleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.īssHII is typically used at 50☌, but is 75% active at 37☌. Sticky ends from different SexAI sites may not be compatible.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence. ![]() Sticky ends from different CsiI sites may not be compatible. Sticky ends from different DraIII sites may not be compatible.įseI gradually loses activity when stored at -20☌. Sticky ends from different KflI sites may not be compatible. PciI is inhibited by nonionic detergents.īsrGI is typically used at 37☌, but is even more active at 60☌.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. Sticky ends from different AlwNI sites may not be compatible.
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